고속 탐색용 유전자회로 기반 단백질 신규 활성 도입

Evolution of enzymes with new specificity by high-throuput screening using DmpR-based genetic circuiuts and multiple flow cytometry rounds Kil Koang Kwon, Dae-Hee Lee, Su Jin Kim, Su-Lim Choi, Eugene Rha, Soo-Jin Yeom, Bindu Subhadra, Jinhyuk Lee, Ki Jun Jeong, Seung-Goo Lee Scientific Reports (2018) 8(1), 2659 DOI: 10.1038/s41598-018-20943-8

Cellulase 고속탐색용 유전자회로 개발 및 신규 효소 발굴

Development of a novel cellulase biosensor that detects crystalline cellulose hydrolysis using a transcriptional regulator Kil Koang Kwon, Soo-Jin Yeom, Dae-Hee Lee, Ki Jun Jeong, Seung-Goo Lee Biochemical and Biophysical Research Communications (2018) 495(1), 1328-1334 DOI: 10.1016/j.bbrc.2017.11.157

신규 CRISPR-Cpf1 (Type V-A) 발굴 및 구동

Efficient Transcriptional Gene Repression by Type V-A CRISPR-Cpf1 from Eubacterium eligens Seong Keun Kim, Haseong Kim, Woo-Chan Ahn, Kwang-Hyun Park, Euijeon Woo, Dae-Hee Lee, and Seung-Goo Lee ACS synthetic Biology (2017) 6(7), 1273-1282 DOI:10.1021/acssynbio.6b00368

CRISPRi 기반 대사흐름 제어 및 유용 대사물질 생산

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli Seong Keun Kim, Wonjae Seong, Gui Hwan Han, Dae-Hee Lee, and Seung-Goo Lee Microbial Cell Factories (2017) 16, 188 DOI:10.1186/s12934-017-0802-x.

메타볼론 기반 효소 활성 개선 기술

Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion Soo-Jin Yeom, Gui Hwan Han, Moonjung Kim , Kil Koang Kwon, Yaoyao Fu, Haseong Kim, Hye-Won Lee, Dae-Hee Lee, Heungchae Jung, and Seung-Goo Lee PLoS One (2017) 12(1):e0170398 DOI: 10.1371/journal.pone.0170398.eCollection

메타볼론 기반 고효율 효소 연속반응 구현

Leucine zipper-mediated targeting of multi-enzyme cascade reactions to inclusion bodies in Escherichia coli for enhanced production of 1-butanol. Gui Hwan Han, Wonjae Seong, Yaoyao Fu, Paul K. Yoon, Seong Keun Kim, Soo-Jin Yeom, Dae-Hee Lee, Seung-Goo Lee Metabolic Engineering (2017) 40, 41-49 DOI: 10.1016/j.ymben.2016.12.012

센서 유전자회로 기반 고효율 맞춤형 미생물 검색 플랫폼

A cell-cell communication-based screening system for novel microbes with target enzyme activities Haseong Kim, Eugene Rha, Wonjae Seong, Soo-Jin Yeom, Dae-Hee Lee, Seung-goo Lee ACS Synthetic Biology (2016) 18;5(11):1231-1238

한국생명공학연구원에 위치한 본 연구실에서는 효소공학, 단백질공학, 대사공학, 시스템생물학 분야의 전문가들과 함께 합성생물학 기반의 원천 기술을 개발하고 관련된 실용화 연구를 수행하고 있습니다.