Publications (first/corresponding only)

Development of a novel cellulase biosensor that detects crystalline cellulose hydrolysis using a transcriptional regulator

Kil Koang Kwon, Soo-Jin Yeom, Dae-Hee Lee, Ki Jun Jeong, Seung-Goo Lee
Biochemical and Biophysical Research Communications (2018) 495(1), 1328-1334
DOI: 10.1016/j.bbrc.2017.11.157

Evolution of enzymes with new specificity by high-throuput screening using DmpR-based genetic circuiuts and multiple flow cytometry rounds

Kil Koang Kwon, Dae-Hee Lee, Su Jin Kim, Su-Lim Choi, Eugene Rha, Soo-Jin Yeom, Bindu Subhadra, Jinhyuk Lee, Ki Jun Jeong, Seung-Goo Lee
Scientific Reports (2018) 8(1), 2659
DOI: 10.1038/s41598-018-20943-8

Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha

Yeom SJ, Kim M, Kim SK, Lee DH, Kwon KK, Lee H, Kim H, Kim DM, Lee SG
BMC Plant Biol. 2018 Jun 15;18(1)

Structural and functional analyses of the cellulase transcription regulator CelR

Yaoyao Fu, Soo-Jin Yeom, Kil Koang Kwon, Jungwon Hwang, Haseong Kim, Eui-Jeon Woo, Dae-Hee Lee and Seung-Goo Lee
FEBS Letters (2018)

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

Seong Keun Kim, Wonjae Seong, Gui Hwan Han, Dae-Hee Lee, and Seung-Goo Lee
Microbial Cell Factories (2017) 16, 188

Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion

Soo-Jin Yeom, Gui Hwan Han, Moonjung Kim , Kil Koang Kwon, Yaoyao Fu, Haseong Kim, Hye-Won Lee, Dae-Hee Lee, Heungchae Jung, and Seung-Goo Lee
PLoS One (2017) 12(1):e0170398
DOI: 10.1371/journal.pone.0170398.eCollection

Leucine zipper-mediated targeting of multi-enzyme cascade reactions to inclusion bodies in Escherichia coli for enhanced production of 1-butanol

Gui Hwan Han, Wonjae Seong, Yaoyao Fu, Paul K. Yoon, Seong Keun Kim, Soo-Jin Yeom, Dae-Hee Lee, Seung-Goo Lee
Metabolic Engineering (2017) 40, 41-49
DOI: 10.1016/j.ymben.2016.12.012

Efficient Transcriptional Gene Repression by Type V-A CRISPR-Cpf1 from Eubacterium eligens

Seong Keun Kim, Haseong Kim, Woo-Chan Ahn, Kwang-Hyun Park, Euijeon Woo, Dae-Hee Lee, and Seung-Goo Lee
ACS synthetic Biology (2017) 6(7), 1273-1282

Tunable control of an Escherichia coli expression system for the overproduction of membrane proteins by titrated expression of a mutant lac repressor

Seong Keun Kim, Dae-Hee Lee, Oh Cheol Kim, Jihyun Kim, and Sung Ho Yoon
ACS Synthetic Biology (2017) 6, 1766-1773

A cell-cell communication-based screening system for novel microbes with target enzyme activities

Haseong Kim, Eugene Rha, Wonjae Seong, Soo-Jin Yeom, Dae-Hee Lee, Seung-goo Lee
ACS Synthetic Biology (2016) 18;5(11):1231-1238

CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production

Seong Keun Kim, Gui-Hwan Han, Wongjae Seong, Haseong Kim, Seon-Won Kim, Dae-Hee Lee, Seung-goo Lee
Metabolic Engineering (2016) 38,228-240

Fermentative production and direct extraction of (-)-α-bisabolol in metabolically engineered Escherichia coli

Gui-Hwan Han, Seong Keun Kim, Paul K. Younghwan Kang, Byoung Su Kim, Yaoyao Fu, Bong Hyun Sung, Heung Chae Jung, Dae-Hee Lee, Seon-Won Kim, Seung-goo Lee
Microbial Cell Factories (2016) 15(1), 185

Highly sensitive and rapid fluorescence detection with a portable FRET analyzer

Haseong Kim, Gui Hwan Han, Yaoyao Fu, Jongsik Gam, Seung-Goo Lee
Journal of Visualized Experiments(JoVE) (2016) 116

Improved metagenome screening efficiency by random insertion of T7 promoters

Yu Jung Kim, Haseong Kim, Seo Hyeon Kim, Eugene Rha, Su-Lim Choi, Soo-Jin Yeom, Hak-Sung Kim, Seung-Goo Lee
Journal of Biotechnology (2016) 230,47-53

Long-term stable and tightly controlled expression of recombinant proteins in antibiotics-free conditions

Soo-Jin Yeom, Dae-Hee Lee, YuJung Kim, Jeongmin Lee, Kil Koang Kwon, Gui-Hwan Han, Haseong Kim, Hak-Sung Kim, Seung-Goo Lee
PLoS One (2016) 11(12):e0166890

Multi-enzyme screening using a high-throughput genetic enzyme screening system

Hseong Kim, Kil Koang Kwon, WonJae Seong, Seung-goo Lee
Journal of Visualized Experiments(JoVE) (2016) 114
DOI: 10.3791/54059

Pseudomonas kribbensis sp. nov., isolated from garden soils in Daejeon, Korea

Dong-Ho Chang, Moon-Soo Rhee, Ji-Sun Kim, Yookyung Lee, Mi Young Park, Haseong Kim, Seung-Goo Lee, Byoung-Chan Kim
Antonie van Leeuwenhoek (2016) 109(11),1433-1446

A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

Dae-Hee Lee, Su-Lim Choi, Eugene Rha, Soo Jin Kim, Soo-Jin Yeom, Jae-Hee Moon and Seung-Goo Lee
BMC Biotechnology (2015) 15,1

A portable FRET analyzer for rapid detection of sugar content

Haseong Kim, Hyo Sang Kim Jae-Seok Ha, Seung-goo Lee
Analyst (2015) 140(10),3384-3389

Ratiometric analyses at critical temperatures can magnify the signal intensity of FRET-based sugar sensors with periplasmic binding proteins

Jongsik Gam, Jae-Seok Ha, Haseong Kim, Dae-Hee Lee, Jeeyeon Lee, Seung-Goo Lee
Biosensors and Bioelectronics (2015) 72,37-43

Controlled localization of functionally active proteins to inclusion bodies using leucine zippers

Su-Lim Choi, Sang Jun Lee, Soo-Jin Yeom, Hyun Ju Kim, Young Ha Rhee, Heung-Chae Jung, Seung-Goo Lee
PLoS One (2014) 9(6),e97093

Toward a generalized and high-throughput enzyme screening system based on artificial genetic circuits

Su-Lim Choi, Eugene Rha, Sang Jun Lee, Haseong Kim, Kil Koang Kwon, Young-Su Jung, Young Ha Rhee, JaeJun Song, Seung-goo Lee
ACS Synthetic Biology (2014) 3(3),163-171

Enzyme-linked Assay of Cellulose-binding Domain Functions from Cellulomonas fimi on Multi-well Microtiter Plate

Hyeon-Dong Kim, Su-Lim Choi, Haseong Kim, Jung Hoon Sohn, and Seung-Goo Lee
Biotechnology and Bioprocess Engineering (2013) 18,575-580

Generating In Vivo Cloning Vectors for Parallel Cloning of Large Gene Clusters by Homologous Recombination

Jeongmin Lee, Eugene Rha, Soo-Jin Yeom, Dae-Hee Lee, Eui-Sung Choi, Seung-Goo Lee
PLoS One (2013) 8(11),e79979

High-throughput screening system based on phenolics-responsive transcription activator for directed evolution of organophosphate-degrading enzymes

Young-Su Jeong, Su-Lim Choi, Hyun-Ho Kyeong, Jin-Hyun Kim, Eui-Joong Kim, Jae-Gu Pan, Eugene Rha, Jae Jun Song, Seung-Goo Lee, and Hak-Sung Kim
Protein Engineering, Design & Selection (2012) 25(11),725-731

Inducible biosynthetic nanoscaffolds as recruitment platforms for detecting molecular target interactions inside living cells

Sangkyu Lee, Jae-Seok Ha, Seung-Goo Lee, and Tae K. Kim
Journal of the American Chemical Society (2012) 134,11346-11349

Mesh-integrated microdroplet array for simultaneous merging and storage of single-cell droplets

Eujin Um, Eugene Rha, Su-Lim Choi, Seung-Goo Lee and Je-Kyun Park
Lab on a Chip (2012) 12(9),1594-1597

Quantitative analyses of individual sugars in mixture using FRET-based biosensors

Jae‐Seok Ha, Jongsik Gam, Su‐Lim Choi, Ki‐Hoon Oh, Hyeon‐Su Ro, Jae Jun Song, Chul Soo Shin and Seung-goo Lee
Biotechnol Progress (2012) 28(5),1376-1383

Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

Su-Lim Choi, Sang Jun Lee, Jae-Seok Ha, Jae Jun Song, Young Ha Rhee, and Seung-Goo Lee
Biotechnology and Bioprocess Engineering (2011) 16,1173-1179

Small-molecule-based nanoassemblies as inducible nanoprobes for monitoring dynamic molecular interactions inside live cells

Sangkyu Lee, Kyoung Hu Lee, Jae-Seok Ha, Seung-Goo Lee, and Tae K. Kim
Angewandte Chemie International Edition (2011) 50,87098713

Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution

Eugene Rha, Sujin Kim, Su-Lim Choi, Seung-Pyo Hong, Moon-Hee Sung, Jae J. Song, Seung-goo Lee
FEBS Journal (2009) 276,6187-6194

Engineering Hybrid Proteins by Modular Recombination and Evolutionary Optimization

Seung-goo Lee, Eugene Rha, Jae-Seok Ha, Jeong-Min Lee, Sun-Hwa Kim
Korean Journal of Microbiology & Biotechnology (2008) 36(2),149-157

Thermostable β-glycosidase-CBD fusion protein for biochemical analysis of cotton scouring efficiency

Jae-Seok Ha, Young-Mi Lee, Su-Lim Choi, Jae Jun Song, Chul-Soo Shin, Ju-Hea Kim, and Seung-Goo Lee
Journal of Microbiology and Biotechnology (2008) 18(3),443-448

Design and application of highly responsive fluorescence resonance energy transfer biosensors for detection of sugar in living Saccharomyces cerevisiae cells

Jae-Seok Ha, Jae Jun Song, Young-Mi Lee, Su-Jin Kim, Jung-Hoon Sohn, Chul-Soo Shin and Seung-Goo Lee
Applied and Environmental Microbiology (2007) 73(22),7408-7414

Functional and structural characterization of thermostable D-amino acid aminotransferases from Geobacillus spp.

Seung-Goo Lee, Seung-Pyo Hong, Jae Jun Song, Su-Jin Kim, Mi-Sun Kwak, and Moon-Hee Sung
Applied and Environmental Microbiology (2006) 72(2),1588-1594

Inactivation of tyrosine phenol-lyase by Pictet-Spengler reaction and alleviation by T15A mutation on intertwined N-terminal arm

Seung-Goo Lee, Seung-Pyo Hong, Do Young Kim, Jae Jun Song, Hyeon-Su Ro and Moon-Hee Sung
FEBS Journal (2006) 273,5564-5573