Seong, Wonjae (성원재)

Post-doc

Synthetic biology
Metabolic engineering
Lab automation
Genetic circuit
Fermentation
Fluorescence-based high-throughput screening
Published

October 30, 2024

Experience

  • 2021.09~, Post.doc, Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) Advisor: Seung-Goo Lee, Haseong Kim

    1. 05~2013. 08, Internship, Synthetic biology & Bioengineering Research center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

Education

  • 2015 ~ 2021, PhD, Department of Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, Republic of Korea

  • 2013 ~ 2015, MS. Department of Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, Republic of Korea

  • 2009 ~ 2012, BS. Department of Bioinformatics and Biotechnology, Korea University, Sejong, Republic of Korea

Research Details

Publications *Co-first authorship - Bak, S. K., Seong, W., Rha, E., Lee, H., Kim, S. K., Kwon, K. K., Kim, H., & Lee, S. G. (2022). Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology. Journal of Microbiology and Biotechnology, 32(8), 1026–1033. https://doi.org/10.4014/jmb.2207.07013

  • Kim, H., Seong, W., Rha, E., Lee, H., Keun, S., Koang, K., … Lee, S. (2020). Biosensors and Bioelectronics Machine learning linked evolutionary biosensor array for highly sensitive and specific molecular identification. Biosensors and Bioelectronics, 170(September), 112670. https://doi.org/10.1016/j.bios.2020.112670

  • Seong, W., Han, G. H., Lim, H. S., Baek, J. I., Kim, S.-J., Kim, D., … Lee, D.-H. (2020). Adaptive laboratory evolution of Escherichia coli lacking cellular byproduct formation for enhanced acetate utilization through compensatory ATP consumption. Metabolic Engineering, 62(September), 249–259. https://doi.org/10.1016/j.ymben.2020.09.005

  • Kim, S. J., Kim, S. K., Seong, W., Woo, S. G., Lee, H., Yeom, S. J., … Lee, S. G. (2019). Enhanced (-)-α-bisabolol productivity by efficient conversion of mevalonate in Escherichia coli. Catalysts, 9(5). https://doi.org/10.3390/catal9050432

  • Kim, S. K., Seong, W*., Han, G. H., Lee, D. H., & Lee, S. G. (2017). CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli. Microbial Cell Factories, 16(1), 1–15. https://doi.org/10.1186/s12934-017-0802-x

  • Hwan, G., Seong, W., Fu, Y., Yoon, P. K., & Keun, S. (2017). Leucine zipper-mediated targeting of multi-enzyme cascade reactions to inclusion bodies in Escherichia coli for enhanced production of 1-butanol. Metabolic Engineering, 40(September 2016), 41–49. https://doi.org/10.1016/j.ymben.2016.12.012

  • Kim, H., Rha, E., Seong, W., Yeom, S. J., Lee, D. H., & Lee, S. G. (2016). A Cell-Cell Communication-Based Screening System for Novel Microbes with Target Enzyme Activities. ACS Synthetic Biology, 5(11), 1231–1238. https://doi.org/10.1021/acssynbio.5b00287

  • Kim, H., Kwon, K. K., Seong, W., & Lee, S.-G. (2016). Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System. Journal of Visualized Experiments : JoVE, (114), 1–7. https://doi.org/10.3791/54059

  • Kim, S. K., Han, G. H., Seong, W., Kim, H., Kim, S. W., Lee, D. H., & Lee, S. G. (2016). CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production. Metabolic Engineering, 38, 228–240. https://doi.org/10.1016/j.ymben.2016.08.006

Selected Conference Presentations

  • Seong, W. Kim, H., Rha, E., Lee, H, Yoon, H., & Lee, S, -G, Bio-part Library Assembly Approach for Organophosphorus Pesticide Biosensor Construction, KSBB Fall meeting and International Symposium 2019, Daegu, Republic of Korea (2019)

  • Kim, H., Rha, E., Kim,S., Seong, W., Lee, H.,& Lee, S.-G., Effective detection of hazardous compounds with biosensor mixture and 3D printed remote monitoring device, KSBB Fall meeting and International Symposium 2019, Daegu, Republic of Korea (2019) (Showcase presentation) - Seong, W., Kim, H., Rha, E., Lee, H. Yoon, H., & Lee, S.-G., Construction of organophosphorus pesticide whole-cell biosensor using bio-part library, KSBB spring meeting and international symposium 2019, Jeju, Republic of Korea (2019) (3-minute speech presentation)

  • Seong, W., Kim, H., Rha, E., Yeom, S. J., Lee, D. H., Lee, H., & Lee, S.-G., High-throughput genetic enzyme screening and identification system, SB 7.0 the seventh international meeting on synthetic biology, Singapore, Singapore (2017) - Seong, W., Kim, H., Lee, H., Kim, S., Yoon, P., & Lee S. –G., Multi-enzyme screening using a high-throughput genetic enzyme screening system, KMB 2016 annual meeting & international symposium, Daejeon, Republic of Korea (2016)

PCT Patent Application

  • Lee, S.–G., Han, G., Seong, W., Lee, D.H., MICROORGANISM CAPABLE OF UTILIZING ACETIC ACID AS SOLE CARBON SOURCE, PCT Application No. PCT/KR2017/010643

  • Lee, S.-G., Rha, E., Kim H., Seong, W., MICROORGANISM PROTOTYPING GENETIC CIRCUIT-BASED SYSTEM FOR SEARCHING FOR NOVEL MICROORGANISM RESOURCES, PCT Application No. PCT/KR2016/007613

  • Lee, S.-G., Kim, H., Seong, W., Yeom, S.J., Lee, D.H., Jung, H.-C., Han, G., PREPARATION OF ENZYME COMPOSITE AND METHOD FOR PRODUCING TARGET MATERIAL USING SAME, PCT Application No. PCT/KR2016/009340

Korea Patent Application

  • 김하성; 이승구; 성원재; 신상; 박상영; 고남일; 최민수, 형질전환 자동화 장치, 출원번호 10-2024-0013338

  • 이승구; 성원재; 김하성; 나유진; 이대희; 권길광; 이혜원; 김성근; 신종혁, 고효율 형질전환 벡터 및 이의 용도, 출원번호 10-2023-0161526

  • 이승구; 성원재; 김하성; 나유진; 이대희; 권길광; 이혜원; 김성근, 유기인계 화합물 감지용 재설계 유전자 회로, 및 이를 기반으로 한 유기인계 화합물의 감지 방법, 출원번호 10-2022-0171593

  • 이승구; 김하성; 나유진; 성원재; 권길광; 염수진; 이대희; 이혜원, 돌연변이 재설계 유전자 회로, 및 이들의 집합체와 기계학습 알고리즘에 기반한 페놀계 화합물의 감지 및 정량방법, 출원번호 10-2019-0153642

  • 이승구; 한귀환; 성원재; 이대희, 아세트산을 유일 탄소원으로 이용할 수 있는 미생물, 출원번호 10-2017-0124176

  • 이승구; 나유진; 김하성; 성원재, 미생물 프로토타이핑 유전자 회로 기반 신규 미생물자원 탐색 시스템, 출원번호 10-2016-0088411 - 이승구; 김하성; 성원재; 염수진; 이대희; 정흥채; 한귀환, 효소복합체의 제조 및 이를 이용한 목적물질의 생산 방법, 출원번호 10-201500119784