Publications since 2020
A highly efficient and versatile genetic engineering toolkit for a methanotrph-based biorefinery
methanotroph
methane
CRISPR
phenol-inducible promoter
mevalonate
CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
CRISPR
mevalonate
antibiotic-free
Novel high-throughput DNA part characterization technique for synthetic biology
DNA parts
circuit design
synthetic biology
long-read sequencing
image analysis
Synthetic 3-UTR valves for optimal metabolic flux control in Escherichia coli
Methane
Methanotroph
Phenol-inducible promoter
CRISPR-base editor
Phosphoketolase
Mevalonate
Synthetic 3-UTR valves for optimal metabolic flux control in Escherichia coli
chemical production
directed evolution
HT screening
yeast
Synthetic 3-UTR valves for optimal metabolic flux control in Escherichia coli
chemical production
directed evolution
HT screening
yeast
Fluorescence-activated cell sorting-mediated directed evolution of Wickerhamomyces ciferrii for enhanced production of tetraacetyl phytosphingosine
chemical production
directed evolution
HT screening
yeast
Single-Cell-Based Screening and Engineering of D‑Amino Acid Amidohydrolases Using Artificial Amidophenol Substrates and Microbial Biosensors
amidase
d-amino acid
artificial substrate
biosensor
HT screening
genetic circuit
Single-Cell-Based Screening and Engineering of D‑Amino Acid Amidohydrolases Using Artificial Amidophenol Substrates and Microbial Biosensors
amidase
d-amino acid
artificial substrate
biosensor
genetic circuit
high-throughput screening
Single cell-based screening and engineering of D-amino acid amidohydrolases using artificial amidophenol substrates and microbial biosensors
high-throughput screening
biosensor
genetic circuit
amidase
Engineering Bacteroides thetaiotaomicron to produce non-native butyrate based on a genome-scale metabolic model-guided design
Bacteroides thetaiotaomicron
Genome-scale metabolic model
Flux-balance analysis
Butyrate
Commensal microbes
Syntrophic co-culture of a methanotroph and heterotroph for the efficient conversion of methane to mevalonate
Co-culture
Methane
Methanotroph
Heterotroph
Mevalonate
Bio-conversion
(−)-α-Bisabolol Production in Engineered Escherichia coli Expressing a Novel (−)-α-Bisabolol Synthase from the Globe Artichoke Cynara cardunculus var. Scolymus
(−)-α-bisabolol
(−)-α-bisabolol synthase
Escherichia coli
fermentation
mevalonate pathway
artichoke
Cβ-Selective Aldol Addition of D‑Threonine Aldolase by Spatial Constraint of Aldehyde Binding
d-threonine aldolase
stereoselectivity
β-hydroxy-α-amino acid
catalytic mechanism
protein engineering
Biosensor-Based Directed Evolution of Methanol Dehydrogenase from Lysinibacillus xylanilyticus
synthetic methylotrophy
methanol dehydrogenase
Lysinibacillus xylanilyticus
biosensor
screening
Antagonistic Control of Genetic Circuit Performance for Rapid Analysis of Targeted Enzyme Activityin Living Cells
inhibitor
antagonist
genetic circuit
phenolic compound
flow cytometry
resistor
Antagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells
genetic circuit
dmpR
high-throughput screening
phenol-inducible promoter
Genetically Encoded Biosensor-Based Screening for Directed Bacteriophage T4 Lysozyme Evolution
bacteriophage T4 lysozyme
genetically encoded biosensor
directed evolution
A designed whole-cell biosensor for live diagnosis of gut inflammation through nitrate sensing
Whole cell biosensor
Gut inflammation
Designer probiotic
Nitrate sensing
Thiosulfate sensing
Boolean AND gate
Machine learning linked evolutionary biosensor array for highly sensitive and specific molecular identification
Genetically-encoded biosensors
Machine learning
Molecular identification
Protein engineering
Biosensor array
High-throughput screening system
Adaptive laboratory evolution of Escherichia coli lacking cellular byproduct formation for enhanced acetate utilization through compensatory ATP consumption
Adaptive laboratory evolution
Escherichia coli
Acetate
Butanol
Mevalonate
Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay
synthetic biology
transcriptional factor-based biosensors
microfluidics
large-scale phenotyping
cell–cell communication
Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device
Assays
Deposition
Genetics
Gold
Hydrogels
Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus
methanol dehydrogenase
rational enzyme engineering
Lysinibacillus xylanilyticus
methylotrophy
methanol oxidation
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