CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli
Microbial Cell Factories
CRISPR interference
Endogenous gene
Escherichia coli
Multiple gene knockdown
n-Butanol
Multiplex control of metabolic pathway genes is essential for maximizing product titers and conversion yields of fuels, chemicals, and pharmaceuticals in metabolic engineering. To achieve this goal, artificial transcriptional regulators, such as clustered regularly interspaced short palindromic repeats (CRISPR) interference (CRISPRi), have been developed to specifically repress genes of interest.
DOI:10.1186/nar/gkac206. IF.(y). Citation . ISSN no.-.